Ecological Health and Freshwater Pathogen Using eDNA Metabarcoding: A Preliminary Assessment for Environmental Surveillance Development in Malaysia.

ABSTRACT: River water enters human life in various ways, with many disease outbreaks closely linked to contaminated sources. This study collected water samples from the Perak River in Malaysia, extracted environmental DNA (eDNA), and analyzed biological communities using metabarcoding and sequencing techniques to assess the local environmental health of the river. Through 16S rRNA sequencing, 4045 bacterial OTUs were identified, while 18S rRNA sequencing revealed 3422 eukaryotic OTUs, highlighting the diverse microbial and eukaryotic communities in the Perak River. The results showed certain organisms such as Serratia marcescens and Strombidium with potentially abnormal abundance, based on comparisons with other studies, suggesting possible organic and heavy metal pollution. Additional

SUBMITTER: Yang J 

PROVIDER: S-EPMC12472127 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature