Proteomic Research of the Stress Response of <i>Saccharomyces cerevisiae W303</i> Yeast to Metal Ions Eluted from Orthodontic Appliances.

ABSTRACT: Although orthodontic appliances are widely used in daily practice, they also have their downsides due to the large amount of metal ions released from their surface. In this study, the influence of such released metal ions on the yeast Saccharomyces cerevisiae W303 as a model organism was investigated. Experimental yeast media in which metal ions (iron, aluminum, nickel, chromium, copper, and manganese) were eluted for 3, 7, 14, and 28 days were prepared and then used for yeast cultivation (up to the early stationary growth phase). The growth, increase, and viability of the cells were tested. The mitochondria were isolated from the spheroplasts, and the mitochondrial proteins were obtained and analyzed by liquid chromatography/mass spectrometry. Fortythree significantly altered prote

SUBMITTER: Dezulovic L 

PROVIDER: S-EPMC12472195 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature