A Novel <i>Lactobacillus acidophilus</i> Strain Isolated from a 2-Month-Old Shiba Inu: In Vitro Probiotic Evaluation Safety Assessment in Mice and Whole-Genome Sequencing Analysis.

ABSTRACT: Owing to their remarkable biological activities and health benefits, probiotics have gained widespread application in enhancing pet health and welfare. Host-derived probiotics are considered optimal due to their unique digestive tract environments. This study isolated Lactobacillus acidophilus L1 from the feces of a 2-month-old Shiba Inu puppy and conducted a comprehensive evaluation of its potential as a probiotic candidate for pet health. Strain L1 demonstrated high tolerance to acidic conditions (survival rates of 90.41%, 92.90% and 98.81% at pH 2, 2.5, and 3.0, respectively) and bile salts (survival rates of 98.05%, 95.68%, and 82.21% at 0.1%, 0.2%, and 0.3% concentrations, respectively). Adhesion to Caco-2 intestinal epithelial cells reached 38.33%, with hydrophobicity of 97.81

SUBMITTER: Huang H 

PROVIDER: S-EPMC12472203 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature