Covalent Organic Framework-Based Nanomembrane with Co-Immobilized Dual Enzymes for Micropollutant Removal.

ABSTRACT: Biocatalytic nanomembranes have emerged as promising platforms for micropollutant remediation, yet their practical application is hindered by limitations in removal efficiency and operational stability. This study presents an innovative approach for fabricating highly stable and efficient biocatalytic nanomembranes through the co-immobilization of horseradish peroxidase (HRP) and glucose oxidase (GOx) within a covalent organic framework (COF) nanocrystal. Capitalizing on the dynamic covalent chemistry of COFs during their self-healing and self-crystallization processes, we achieved simultaneous enzyme immobilization and framework formation. This unique confinement strategy preserved enzymatic activity while significantly enhancing stability. HRP/GOx@COF biocatalytic membrane was prepared t

SUBMITTER: Zhao J 

PROVIDER: S-EPMC12472217 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature