A Novel Schiff Base Probe Based on Fluorescein for Fluorometric and Colorimetric Dual-Mode Rapid Detection of Cu².

ABSTRACT: Copper is an important environmental pollutant that poses a significant threat to human health and environmental safety. Therefore, the development of methods for detecting Cu²⁺ is of great significance. A novel fluorometric/colorimetric dual-mode sensor for detecting Cu²⁺ was synthesized by Schiff base reaction using fluorescein hydrazide and 8-hydroxyjulonidine-9-carboxaldehyde as raw materials. Cu²⁺ could form a complex with the probe in a stoichiometric ratio of 1:1. Within 1 min, the fluorescence of the probe rapidly quenched at 540 nm, and the absorbance reached a stable state at 452 nm. The color of the solution changed from light yellow to yellow, achieving real-time and visual detection of Cu²⁺. This probe exhibited exceptional selectivi

SUBMITTER: Yang Z 

PROVIDER: S-EPMC12472230 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature