Thymidine-Inosine Dimer Building Block for Reversible Modification of Synthetic Oligonucleotides.

ABSTRACT: Modification of synthetic oligonucleotides and DNA is widely used in many applications in the life sciences. However, in most cases, modified DNA cannot be restored to its native state. Here, we report the preparation of a thymidine-inosine dimer building block (TID) for oligonucleotide synthesis. The TID modification supports the functionalization of synthetic oligonucleotides, which can later be removed to restore the DNA strand to its native state. The TID unit allows for a wide spectrum of postsynthetic modifications of oligonucleotides through click chemistry, including conjugation with fluorescent tags and small molecules, preparation of branched oligonucleotide scaffolds, and anchoring to a solid support. Due to the modification of the thymine base, the TID unit reduces the stabilit

SUBMITTER: Kolganova NA 

PROVIDER: S-EPMC12472319 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature