Intracellular Dual Behavior of Trolox in HeLa Cells and 3T3 Fibroblasts Under Basal and H₂O₂-Induced Oxidative Stress Conditions.

ABSTRACT: Trolox, a water-soluble analog of vitamin E, is widely used as a reference antioxidant in in vitro biochemical assays. However, its intracellular redox behavior is known to vary depending on both concentration and oxidative context. In this study, we investigated the dose-dependent antioxidant and prooxidant effects of Trolox in two cellular models, HeLa cells and 3T3 cells exposed for 1 h to increasing concentrations (2-160 µM), under both basal conditions and oxidative stress induced by hydrogen peroxide. Intracellular oxidative changes were assessed using the oxidative stress-sensitive fluorescent probe CM-H₂DCFDA. Under basal conditions, Trolox exerted slight dose-dependent antioxidant behavior in 3T3 cells on the basal production of ROS in concentrations ranging from 2 µM t

SUBMITTER: Giordano ME 

PROVIDER: S-EPMC12472327 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature