Indazol-Pyrimidine Hybrids: Design, Synthesis, and Antiproliferative Activity Against Human Cancer Cell Lines.

ABSTRACT: The current study outlines a synthetic method for creating a new class of indazol-pyrimidine derivatives 4a-h and 5a-h. The new derivatives were evaluated as in vitro cytotoxic agents against three types of cancer cell lines (MCF-7, A549 and Caco-2), utilizing the MTT assay. Compounds 4a, 4c, 4d, 5a and 5f demonstrated potent cytotoxic activity against MCF-7 cell line, showing higher activity than the reference drug Staurosporine. Among the examined compounds, 5f showed a strong cytotoxic effect against all three tested cancer cells (MCF-7, A549 and Caco-2), with IC₅₀ values of 1.858, 3.628 and 1.056 µM, respectively. In comparison, the reference drug exhibited IC₅₀ values of 8.029, 7.354 and 4.202

SUBMITTER: Al-Tuwaijri HM 

PROVIDER: S-EPMC12472346 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature