Bioprospecting of Goat Rumen Microbiota for Optimum Cellulase Enzyme Production to Support Sustainable Bioenergy Systems.

ABSTRACT: This study reports the isolation and optimization of cellulase-producing bacteria from the gastrointestinal tract of South African goats for the pretreatment of lignocellulosic biomass in bioenergy applications. Among the isolates, three strains, Bacillus KC50, Bacillus KC70, and Proteus mirabilis KC94, were identified by 16S rDNA sequencing. To our knowledge, this is the first report of cellulolytic optimization in P. mirabilis derived from goat rumen. Enzyme production was optimized under varying pH, temperature, and incubation conditions. P. mirabilis KC94 exhibited robust enzyme activity at pH 7 and 35 °C, with stability across a broader range than the Bacillus strains. Peak activity occurred at 84 h of incubation, reflecting strain-specific me

SUBMITTER: Rabapane KJ 

PROVIDER: S-EPMC12472465 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature