Role of NaCl and Glutamine on Biofilm Production from <i>Pseudomonas aeruginosa</i>.

ABSTRACT: Pseudomonas aeruginosa is an opportunistic pathogen capable of forming antibiotic-resistant biofilms, contributing to persistent infections and treatment failure. Environmental factors such as osmolarity and nutrient availability are known to influence biofilm formation and virulence. In this study, we investigated the effects of NaCl depletion and glutamine supplementation on biofilm production in three P. aeruginosa strains: the laboratory strain ATCC 27853 and two clinical isolates with distinct antibiotic resistance profiles and phenazine production patterns (P. aeruginosa Pr, pyorubrin-producing, and P. aeruginosa Pc, pyocyanin-producing). Bacteria were cultured in standard Luria-Bertani (LB) medium, LB without NaCl, and LB in which yeast extract was replac

SUBMITTER: De Plano LM 

PROVIDER: S-EPMC12472604 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature