The Impacts of Essential Gcp/TsaD Protein on Cell Morphology, Virulence Expression, and Antibiotic Susceptibility in <i>Staphylococcus aureus</i>.

ABSTRACT: Our previous studies identified the Gcp/TsaD protein as essential for Staphylococcus aureus survival and implicated it in tRNA modification. Here, we demonstrate its broader role in bacterial physiology. Through a morphological analysis, RNA sequencing, network-based bioinformatics, and antibiotic susceptibility testing, we show that Gcp/TsaD influences cell morphology, cell wall integrity, transcriptional regulation, virulence, and antibiotic response. Gcp/TsaD depletion caused reduced cell size and increased cell wall thickness, suggesting its roles in cell division and peptidoglycan biosynthesis. The kinetic transcriptomic analysis revealed widespread changes in gene expression, particularly in the translation and amino acid biosynthesis pathways, supporting its function in maint

SUBMITTER: Guo H 

PROVIDER: S-EPMC12472773 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature