Implementation of a Novel Nanobody Panel for the Efficient Capture of Extracellular Vesicles from Human Plasma.

ABSTRACT: Extracellular vesicles (EVs) are nanoscale particles released by cells and are significant components in intercellular communication. Their ability to reflect the molecular state of parental cells and their presence in body fluids make them increasingly recognized as promising non-invasive biomarkers for different pathological conditions. However, the existence of different EV populations and frequent co-isolation of contaminants present challenges for EV purification and downstream analyses. In this study, we used three novel nanobodies (VHH) for selective isolation of EVs from human plasma. Nanobodies were obtained by direct panning on EVs. All examined nanobodies have excellent physicochemical properties resulting in excellent expression and solubility. The three nanobodies being studie

SUBMITTER: Tursunovic M 

PROVIDER: S-EPMC12472977 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature