Nagilactone C from the Seeds of <i>Podocarpus nakaii</i> May Protect Against LPS-Induced Acute Lung Injury via STAT Signaling Pathway Inhibition.

ABSTRACT: Background/Objectives: Acute lung injury (ALI) is a respiratory disorder lacking specific targeted therapy. Our preliminary screening revealed that the ethanol extract of the seeds of Podocarpus nakaii (EESPN) alleviated the symptoms of ALI in mice. The objectives of this study were to identify the active constituents in EESPN and study the mechanism involved. Methods: Column chromatography was performed to separate the chemical constituents of EESPN. The structures of the isolates were determined via spectroscopic methods. MTT assays, Western blotting, histological analysis, TUNEL assays, immunofluorescence staining, transcriptomic analysis, and quantitative real-time polymerase chain reaction (qRT-PCR) were employed to evaluate the anti-inflammatory activity and to e

SUBMITTER: Chen X 

PROVIDER: S-EPMC12472992 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature