Reverse Transcription Recombinase-Aided Amplification Assay for Newcastle Disease Virus in Poultry.

ABSTRACT: Newcastle disease (ND) is a highly contagious and economically significant viral infection that affects poultry globally, with recurrent outbreaks occurring even among vaccinated flocks in Egypt. Caused by the Newcastle disease virus (NDV), the disease results in substantial losses due to high mortality rates, decreased productivity, and the imposition of trade restrictions. This study aimed to develop a rapid, sensitive, and field-deployable diagnostic assay based on real-time reverse transcription recombinase-aided amplification (RT-RAA) for the detection of all NDV genotypes in clinical avian specimens. Primers and an exo-probe were designed based on the most conserved region of the NDV matrix gene. After testing ten primer combinations, the pair NDV RAA-F1 and RAA-R5 demonstrated the h

SUBMITTER: Yehia N 

PROVIDER: S-EPMC12473048 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature