Proteomic Insights into the Interaction of Chitosan Nanoparticles with Canine MDCK Epithelial Cells.

ABSTRACT: Chitosan is considered an excellent biomaterial for epithelial healing treatment. However, information on its molecular interaction with cells at a molecular level is still lacking. Thus, in the present study, homemade synthesized chitosan nanoparticles (CS NPs) and their physicochemical characterization were examined; we found that NPs had average sizes of 15, 30, and 125 nanometers by modifying some variables in the synthesis protocols. It is worth noting that a crystalline structure was found on the smallest NPs, with an average size of 15 nm, as observed in high-resolution transmission electron micrographs. To study the in vitro interaction of CS NPs with Madin-Darby canine kidney (MDCK) cells, co-culturing was performed, and cell viability was assessed. We found that NPs were not toxi

SUBMITTER: Galvan-Flores LE 

PROVIDER: S-EPMC12473073 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature