5-AzaC Facilitates Somatic Embryogenesis and Germination Across Two Embryogenic Lines in <i>Larix olgensis</i>.

ABSTRACT: Long-term subculture of embryogenic callus leads to a decline in somatic embryogenesis and germination capacity, which may be associated with increased methylation levels. 5-Azacytidine (5-AzaC), a methylation inhibitor, modulates DNA methylation and is widely involved in regulating plant growth, development, and metabolism. In order to investigate the effect of 5-AzaC on somatic embryogenesis and germination in Larix olgensis, we supplemented the proliferation medium with different concentrations of 5-AzaC. The results showed that the addition of 5-AzaC inhibited the proliferation of embryogenic callus, with the proliferation of embryogenic line N2 completely inhibited at 100 μM, while that of embryogenic line N4 ceased at 20 μM. In contrast, treatment with 10 μM and 20 μM of 5-Aza

SUBMITTER: Zhao W 

PROVIDER: S-EPMC12473665 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature