Green Synthesis of Zwitterionic-Cyclodextrin Hybrid Polymer for Efficient Extraction of Polypeptides: Combination of Instrumental Analysis and DFT Calculation.

ABSTRACT: Adhering to the principles of green analytical chemistry (GAC) is crucial for advancing sample pretreatment. In this work, we developed a green in-tube solid-phase microextraction (IT-SPME) material utilizing non-toxic cyclodextrin and zwitterionic polymers as co-functioning monomers. The hybrid monolithic material was synthesized within 38 min via an efficient epoxy ring-opening reaction and free radical polymerization. Comprehensive characterization confirmed a rigid framework with strong anti-swelling properties, good permeability, and high enrichment efficiency on the polymers. When coupled with HPLC-UV, the optimized IT-SPME method enabled highly sensitive detection of polypeptides (vancomycin and teicoplanin) in aqueous matrices, achieving detection limits as low as 15.0-20.0 μg L

SUBMITTER: Lei X 

PROVIDER: S-EPMC12473841 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature