Identification of Variable Lymphocyte Receptors That Target the Human Blood-Brain Barrier.

ABSTRACT: Background/Objectives: Receptor-mediated transcytosis utilizing the native transporters at the blood-brain barrier (BBB) is a growing strategy for the delivery of therapeutics to the brain. One of the major challenges in identifying appropriate human transcytosis targets is that there is a species-specific transporter expression profile at the BBB, complicating translation of successful preclinical candidates into humans. In an effort to overcome this obstacle and identify proteins capable of binding human-relevant BBB ligands, we generated and screened a BBB-targeting library against human-induced pluripotent stem cell-derived brain microvascular endothelial-like cells (iPSC-derived BMEC-like cells). As targeting molecules, we used lamprey antibodies, known as variable lymphocyte r

SUBMITTER: Katt ME 

PROVIDER: S-EPMC12473985 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature