Comprehensive Insight into Microcystin-Degrading Mechanism of <i>Sphingopyxis</i> sp. m6 Based on Mlr Enzymes.

ABSTRACT: Bacterial degradation is one important Microcystin (MC) removal method in the natural environment. The traditional MC-degrading pathway was proposed based on the functions of individual recombinant Mlr enzymes and the structures of the main MC-degrading products. However, the actual MC-degrading mechanism by Mlr enzymes in wild-type bacteria remains unclear. In this study, bioinformatic analysis, heterologous expression, and knockout mutation were performed to elaborate the MC-degrading mechanism by Mlr enzymes in Sphingopyxis sp. m6. The results showed that mlr gene cluster was initially acquired by horizontal gene transfer, followed by vertical inheritance within Alphaproteobacteria. Mlr enzymes exhibit distinct subcellular localizations and possess diverse conserved

SUBMITTER: Ding Q 

PROVIDER: S-EPMC12474180 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature