Utilizing Multiple Behavioral Endpoints to Identify Negative Control Chemicals in a Larval Zebrafish Behavior Assay.

ABSTRACT: Identifying reliable negative control compounds is essential for determining the sensitivity and specificity of screening assays. However, well-characterized negative controls for developmental neurotoxicity behavioral assays in larval zebrafish (Danio rerio) are lacking. This study evaluated nine chemicals with no reported evidence of mammalian developmental neurotoxicity, and a positive control (fluoxetine) for developmental and neurodevelopmental (i.e., behavioral) toxicity in zebrafish. Embryos were exposed to each chemical (≤100 µM) during development, 0-5 days post-fertilization (dpf), then assessed as larvae (6 dpf) using a locomotor behavior light-dark transition test. Behavior was analyzed using two methods: (1) the traditional method, comparing the average total distance m

SUBMITTER: Knapp BR 

PROVIDER: S-EPMC12474258 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature