Development of a Microfluidic Point-of-Care Platform for HPV Detection Based on Helicase-Dependent Amplification.

ABSTRACT: Human papillomavirus (HPV) is the most prevalent sexually transmitted infection worldwide and a leading cause of cervical cancer, accounting for over 300,000 deaths annually, primarily due to high-risk genotypes HPV-16 and HPV-18. Conventional molecular diagnostic methods, such as polymerase chain reaction (PCR), require expensive instrumentation and well-equipped laboratories, which limits their applicability in low-resource or decentralized settings. To address this challenge, the aim of this study was to develop a prototype point-of-care (POC) diagnostic platform based on helicase-dependent amplification (HDA) integrated into a microfluidic device for the specific detection of HPV-16 and HPV-18. The proposed POC platform comprises a disposable poly (methyl methacrylate) (PMMA) microflui

SUBMITTER: Gonzalez-Gonzalez E 

PROVIDER: S-EPMC12474266 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature