Rapid Detection of Saxitoxin Using a Nucleic Acid Aptamer Biosensor Based on Graphene Oxide as a Fluorescence Quencher.

ABSTRACT: Saxitoxin (STX) is a toxin with paralyzing and lethal properties, necessitating the development of a simple analytical method. This study developed a nucleic acid aptamer biosensor using graphene oxide (GO) as a fluorescence quencher for STX detection. GO was combined with M30-f, an STX nucleic acid aptamer modification with 5-carboxyfluorescein, which can produce fluorescence absorption under the conditions of an excitation wavelength of 408 nm and emission wavelength of 515 nm. Based on the principle of fluorescence resonance energy transfer, the fluorescence of M30-f was quenched. In the presence of STX, M30-f specifically binds to STX and dissociates from the GO surface, thereby restoring fluorescence. The STX content can be quantitatively detected through differences in fluorescence a

SUBMITTER: Jiao Y 

PROVIDER: S-EPMC12474413 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature