Comparative Performance of Digital PCR and Real-Time RT-PCR in Respiratory Virus Diagnostics.

ABSTRACT: Background: Respiratory viral infections pose a major global health burden, and molecular diagnostics such as Real-Time RT-PCR have revealed frequent co-infections. However, precise quantification of viral RNA remains challenging. Digital PCR (dPCR) offers absolute quantification without standard curves and may improve diagnostic accuracy. This study compares dPCR and Real-Time RT-PCR in detecting and quantifying influenza A, influenza B, respiratory syncytial virus (RSV), and SARS-CoV-2 during the 2023-2024 tripledemic. Methods: A total of 123 respiratory samples were analysed and stratified by cycle threshold (Ct) values into high, medium, and low viral load categories. Both dPCR and Real-Time RT-PCR were used to quantify and compare viral loads across these categories.

SUBMITTER: Bianconi I 

PROVIDER: S-EPMC12474457 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature