Preparation, expression, and anti-cancer effects of the novel fusion protein rONC-T7-pHLIP.

ABSTRACT: Onconase (ONC), a novel antitumor protein, exhibits significant cytotoxic effects on various tumor cells. Although recent advancements have been made in ONC-based molecularly targeted drugs, most are single-targeting proteins with limitations such as poor targeting precision and low efficiency. In this study, the fusion protein rONC-T7-pHLIP was constructed by linking ONC with the T7 peptide, which specifically recognizes the transferrin receptor, and the pHLIP peptide, which actively targets the acidic tumor microenvironment, using a flexible linker peptide (GGGGS)₃. The engineered strain E. coli BL21(DE3) / rONC-T7-pHLIP was used for single-factor analysis of IPTG concentration, induction time, and induction temperature, followed by orthogonal experimental design to optimize t

SUBMITTER: Yang G 

PROVIDER: S-EPMC12474709 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature