Integrated multi-omics analysis reveals PTM networks as key regulators of colorectal cancer progression and immune evasion.

ABSTRACT:

Background

Colorectal cancer (CRC) is a leading cause of cancer mortality, with treatment resistance often driven by molecular heterogeneity and an immunosuppressive tumor microenvironment (TME). Post-translational modifications (PTMs) regulate key oncogenic processes, but their comprehensive role in CRC progression and immune evasion remains unexplored.

Methods

We integrated multi-omics data from bulk RNA-seq (GEO/TCGA, n = 1,783), single-cell transcriptomics (41,143 cells), and Mendelian randomization. Differential expression, GSVA, and machine learning (LASSO/SVM/Random Forest) were used to identify PTM-associated signatures. Functional validation included spatial transcriptomics, and immune profiling.

Results

Multi-omics analysis identified dysregulation in 80% of

SUBMITTER: Yang G 

PROVIDER: S-EPMC12474747 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature