EGFL9 regulates the growth and metastasis of hepatocellular carcinoma through EGFR/PI3K/AKT signaling pathway and amino acid modulation.

ABSTRACT:

Background

Previous research has highlighted the roles of epidermal growth factor-like (EGFL) 7 and EGFL8 in hepatocellular carcinoma (HCC), while EGFL9 has been implicated in other cancers such as breast cancer. Despite this, its role in HCC remains unexplored. This study aims to investigate EGFL9's impact on HCC progression.

Methods

EGFL9 expression was evaluated in 30 HCC tissue samples and 5 HCC cell lines with different metastatic potentials using reverse transcription quantitative polymerase chain reaction (RT-qPCR). Serum EGFL9 levels were measured in 30 HCC patients via ELISA. EGFL9 knockdown and overexpression HCC cell lines were established, and a series of assays including MTT, apoptosis, wound healing, Transwell, subcutaneous tumor formation, and tail vein inject

SUBMITTER: Li Z 

PROVIDER: S-EPMC12474750 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature