Mechanisms of Saposhnikovia divaricata in attenuating NaAsO₂-induced neural injury via the PI3K/AKT pathway.

ABSTRACT: Saposhnikovia divaricata (S. divaricata) has demonstrated significant efficacy in mitigating arsenic poisoning. However, its protective effects of S. divaricata against NaAsO₂-induced neural injury remain unclear. Based on network pharmacology, through intersection analysis, the common targets of the effective ingredients and targets of S. divaricata were selected from the TCMSP database, and the targets related to neural injury and arsenic toxicity were selected from the GeneCards database. Subsequently, protein-protein interaction (PPI), GO and KEGG enrichment analyses were carried out. We validated the PI3K/AKT pathway in vitro by assessing drug effects by CCK8 assays, flow cytometry, Western blot, and RT-qPCR. The PI3K/AKT pathway was screened by using network pharmacology,

SUBMITTER: Gu D 

PROVIDER: S-EPMC12474866 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature