V-ATPase-dependent induction of selective autophagy.

ABSTRACT: The general consensus is that the vacuolar-type H⁺-translocating ATPase (V-ATPase) is critical for macroautophagy/autophagy. However, there is a fundamental conundrum because follicular lymphoma-associated mutations in the V-ATPase result in lysosomal/vacuolar deacidification but elevated autophagy activity under nutrient-replete conditions and the underlying mechanisms remain unclear. Here, working in yeast, we show that V-ATPase dysfunction activates a selective autophagy flux termed "V-ATPase-dependent autophagy ". By combining transcriptomic and proteomic profiling, along with genome-wide suppressor screening approaches, we found that V-ATPase-dependent autophagy is regulated through a unique mechanism distinct from classical nitrogen starvation-induced autophagy. Tryptophan

SUBMITTER: Huang Y 

PROVIDER: S-EPMC12474928 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature