Effects of Slit2 on hypertrophic scar formation: an in vitro study in fibroblasts.

ABSTRACT: Hypertrophic scarring (HTS) is a common complication of burns and has been associated with Slit2 protein. Slit2 plays various physiological and pathological roles in some body organs and tumors. However, its role in HTS development remains poorly understood. Therefore, this study investigated the involvement of intracellular and exogenous Slit2 in hypertrophic scar formation in vitro. Human normal fibroblasts (HNFs) and hypertrophic scar fibroblasts (HTSFs) were isolated from normal skin and hypertrophic scar tissues, respectively. Immunohistochemistry was conducted, and molecular expressions were detected by real-time polymerase chain reaction and western blotting. Cell proliferation and mobility were assessed. The results showed that Slit2 and Robo1 expressions were higher in the dermis

SUBMITTER: Cui HS 

PROVIDER: S-EPMC12474968 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature