Enhancing developmental potential of vitrified in vitro matured bovine oocytes using extracellular vesicles from large follicles.

ABSTRACT: This study investigates the differential effects of extracellular vesicles (EVs) derived from bovine follicular fluid (FF) on bovine oocytes cryotolerance, focusing on EVs from small (3-5 mm) and large (> 9 mm) follicles, with the aim of optimizing vitrification protocols. FF-EVs were isolated and characterized for size and concentration. Oocytes were in vitro matured with FF-EVs prior to vitrification. Post-warming assessment included spindle morphology, DNA integrity, embryo development, total cell count, and apoptotic index of resulting blastocysts. EVs uptake by cumulus-oocyte complexes was confirmed via confocal microscopy. While percentages of oocytes at the metaphase II stage were similar across groups, vitrification significantly reduced normal spindle configurations, except in ooc

SUBMITTER: Diaz-Munoz J 

PROVIDER: S-EPMC12475026 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature