A conserved long-range RNA interaction in SARS-CoV-2 recruits ADAR1 to enhance virus proliferation.

ABSTRACT: Long-range RNA-RNA pairing impacts the genome structure and function of SARS-CoV-2 variants. To understand the structure and function relationships of different SARS-CoV-2 variants that have emerged during the COVID-19 pandemic, we perform high-throughput structure probing and modelling of the genomic structures of the wildtype (WT), Alpha, Beta, Delta and Omicron variants of SARS-CoV-2. We observe that genomes of SARS-CoV-2 variants are generally structurally conserved, and that single-nucleotide variations and interactions with RNA binding proteins can impact RNA structures across the viruses. Importantly, using proximity ligation sequencing, we identify many conserved ultra-long-range RNA-RNA interactions, including one that spans more than 17 kb in both the WT virus and the Omicron var

SUBMITTER: Yang SL 

PROVIDER: S-EPMC12475128 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature