Hypersonic levitation and spinning: paving the way for enhanced single-cell analysis via contactless tissue dissociation.

ABSTRACT: In the realm of single-cell analysis, effective tissue dissociation is a cornerstone yet often hampered by the drawbacks of traditional methods. Mechanical and enzymatic dissociation methods suffer from long processing times, reduced cell viability, and the loss of rare cell populations. Herein, we introduce a revolutionary tissue dissociation approach, Hypersonic Levitation and Spinning (HLS), which capitalizes on a uniquely designed triple-acoustic resonator probe. This probe enables the target tissue sample to levitate and execute a 'press-and-rotate' operation within a confined flow field, generating microscale 'liquid jets' that exert precise hydrodynamic forces in a non-contact manner. Through this mechanism, the shear forces on the tissue are enhanced, facilitating rapid and efficie

SUBMITTER: Bai Y 

PROVIDER: S-EPMC12475160 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature