Identification of best housekeeping gene for normalization in qRT-PCR data under different development stages and stress conditions in Vigna mungo.

ABSTRACT: The qRT-PCR has emerged as the most sought-after local gene expression profiling technique. The absolute quantification of the mRNA is highly affected by the handling artifacts. In this regard, the internal control genes serve a vital role in negating the handling errors. So far, the most stable housekeeping genes to be used for the normalization of qRT-PCR data are lacking in V. mungo, commonly known as urdbean or blackgram. Therefore, in the current study we aimed to explore the stability of the UFO, TUB2, ACT2, RHA, ISTL6, CyP, DNAJ, RBCS3B, RPS34, USP21, USB1, EfTu, UBQ10, and UBQE9 genes of V. mungo in 17 different developmental stages and 4 different abiotic stresses. The well-acknowledged algorithms such as geNorm, NormFinder, BestKeeper, the comparative ΔCt method and comprehensive

SUBMITTER: Jha SK 

PROVIDER: S-EPMC12475163 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature