Characterizing interactions of Staphylococcus aureus and Escherichia coli in dual-species implant-associated biofilms.

ABSTRACT: While Staphylococcus aureus is the predominant pathogen in periprosthetic joint infections (PJI), polymicrobial infections involving Gram-negative organisms, such as Escherichia coli, complicate clinical outcomes. Little is known regarding implant-associated polymicrobial interactions; consequently, current PJI treatments are not optimized for their treatment. This study explored the dynamics of S. aureus-E. coli dual-species biofilms, focusing on biofilm properties, antibiotic susceptibility, and molecular interactions. Co-culture experiments revealed that E. coli significantly suppressed S. aureus biofilm viability, observed for methicillin-susceptible S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA). Microscopic analyses demonstrated enhanced E. coli attachment facilitated by

SUBMITTER: Sekar A 

PROVIDER: S-EPMC12475442 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature