Recombinant production of canine vitronectin for optimizing the culture of canine induced pluripotent stem cells.

ABSTRACT:

Introduction

Canine induced pluripotent stem cells (ciPSCs) have attracted attention as valuable tools in veterinary regenerative medicine and disease modeling. Feeder-free culture of ciPSCs using iMatrix-511 has become feasible. Since the choice of extracellular matrix (ECM) has been shown to significantly affect not only the maintenance of pluripotency but also the efficiency of directed differentiation, systematic evaluation of multiple ECM substrates is considered important in ciPSC culture as well. Furthermore, considering future clinical applications, it is essential to establish a xeno-free culture system. Vitronectin (VTN) is a protein that can be easily expressed as a recombinant product in Escherichia coli, making it suitable for scalable ECM production. In this st

SUBMITTER: Shishida K 

PROVIDER: S-EPMC12475865 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature