Multimodal retinal imaging by visible light optical coherence tomography and phosphorescence lifetime ophthalmoscopy in the mouse eye.

ABSTRACT:

Significance

Oxygen metabolism is important to retinal disease development, but current imaging methods face challenges in resolution, throughput, and depth sectioning to spatially map microvascular oxygen.

Aim

We aim to develop a multimodal system capable of simultaneous phosphorescence lifetime imaging scanning laser ophthalmoscopy (PLIM-SLO) and visible light optical coherence tomography (VIS-OCT) to capture capillary-level oxygen partial pressure ( pO2 ) and structural volumes in rodents.

Approach

C57BL/6 mice were imaged by VIS-OCT with high-definition (10 kHz raster) and Doppler (100 kHz circular) protocols. Phosphorescent probe Oxyphor 2P was retro-orbitally injected to enable intravascular PLIM-SLO imaging ( 200  μs pixel dwell time), and a tunable lens was us

SUBMITTER: Nolen S 

PROVIDER: S-EPMC12476265 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature