ECM Protein CYR61 Promotes Migration and Osteoblastic Differentiation of Irradiation BMSCs via Migrasomes.

ABSTRACT: Osteoradionecrosis of the jaw (ORNJ) is a complication of radiation therapy that can lead to hard-to-repair bone defects. Bone marrow mesenchymal stem cells (BMSCs) have been identified as potential "seeds" for restoring bone defects. In this study, we reported extracellular matrix protein cysteine-rich angiogenic inducer 61 (CYR61) to enhance the migratory and osteogenic functions of irradiated BMSCs (IR BMSCs) by migrasomes. Various assays, including alkaline phosphatase (ALP) activity assay, Cell Counting Kit-8 (CCK-8), apoptosis analysis, qRT-PCR, western blot, ALP staining, alizarin red S (ARS) staining, wound healing assay, transwell assay, and co-immunoprecipitation (co-IP) were conducted to assess the optimal radiation dose for generating IR BMSCs and migrasome functionality. Prote

SUBMITTER: Yan C 

PROVIDER: S-EPMC12476934 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature