Establishment and application of a dual chip digital PCR assay for detection of PDCoV and PEDV.

ABSTRACT:

Introduction

Coinfection with porcine deltacoronavirus (PDCoV) and porcine epidemic diarrhea virus (PEDV) is a major cause of acute diarrhea in piglets, which poses a significant challenge to the swine industry. The early detection and control of these two viruses require highly sensitive diagnostic tools. We developed a novel chip digital PCR (cdPCR) assay that uses two probes for the simultaneous quantitative detection of both PDCoV and PEDV in clinical samples.

Methods

In this study, the dual cdPCR reaction system, including the annealing temperature and primer-probe concentration ratio, was systematically optimized. Additionally, we validated the developed method for specificity, sensitivity, linearity, and repeatability. Finally, the method was applied to assess the bio

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12477013 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature