Distant metastases of melanoma exhibit varying extent of intrapatient proteogenomic heterogeneity.

ABSTRACT:

Background

Metastatic melanoma is a highly aggressive disease with poor survival rates despite recent therapeutic advancements with immunotherapy. The proteomic landscape of advanced melanoma remains poorly understood, especially regarding proteomic heterogeneity across metastases within patients.

Methods

We collected 83 melanoma metastases from 19 different metastatic sites in 24 patients with advanced metastatic melanoma almost exclusively from the pre-immunotherapy era, using semi-rapid autopsies. The metastases were subjected to histopathological evaluation, RNA-sequencing and mass spectrometry-based proteomics for protein quantitation and non-reference peptide (NRP) sequence detection using a proteogenomic data integration approach.

Results

NRPs associated with m

SUBMITTER: Szeitz B 

PROVIDER: S-EPMC12477065 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature