<i>sodA</i> modulates <i>in vitro</i> and <i>in vivo</i> virulence of <i>Yersinia enterocolitica</i>.

ABSTRACT:

Introduction

Yersinia enterocolitica is a significant foodborne Gram-negative pathogen causing gastroenteritis and systemic infections. Its virulence is attributed to its ability to withstand oxidative stress and evade host immunity. This study investigates the role of the sodA gene, which encodes manganese-dependent superoxide dismutase (Mn-SOD), in the pathogenicity of Y. enterocolitica.

Methods

Using sodA knockout (ΔsodA) and complemented (ΔsodA^C ) strains, we assessed bacterial adhesion and invasion in Caco-2 epithelial cells, intracellular survival in RAW264.7 macrophages, and colonization in BALB/c mice. Inflammatory responses were evaluated through histopathology, immunohistochemistry (NF-κB p65), and qRT-PCR analysis of cytokine

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12477148 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature