Untargeted metabolomics based on HS- SPME-GC-MS revealing the dynamic evolution of aroma components during cigar aging.

ABSTRACT: Cigar aging plays a critical role in enhancing flavor complexity and overall quality of cigar. In this study, an untargeted metabolomics method based on headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS) was used to comprehensively profile volatile compounds and track their dynamic changes during aging. A total of 1,836 volatile compounds were identified, primarily comprising heterocyclics, terpenoids, ketones, and esters. Multivariate analyses, including principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA), segmented the aging process into four distinct stages, with most differential metabolites showing upregulated trends. Notably, terpenoids exhibited substantial increases in both dive

SUBMITTER: Yang M 

PROVIDER: S-EPMC12477199 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature