Bioengineering a Patient-Derived Vascularized Lung Tumor-on-Chip Model to Decipher Immunomodulation by the Endothelium.

ABSTRACT: The endothelium compartment is a key player in tumor initiation and progression, but most existing tumor-on-chip models lack clinical relevance. Here, a 3D vascularized tumor-on-chip (vToC) model, generated with patient-derived microvascular endothelial cells (ECs) that are freshly isolated from surgical lung cancer samples, is presented. The microvessel molecular identity, morphology, and functionality are assessed by transcriptomic, immunofluorescence, TNF-α stimulation, and permeability assays. Lung cancer cells, cancer-associated fibroblasts (CAFs), and CD8+ tumor-infiltrating lymphocytes are embedded into the surrounding collagen matrix to partially recapitulate the lung tumor microenvironment (TME). The proof-of-concept of feasibility to generate personalized immunocompetent vToC com

SUBMITTER: Lansche C 

PROVIDER: S-EPMC12477576 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature