Metagenome-inspired libraries to engineer phage M13 for targeted killing of Gram-negative bacterial species.

ABSTRACT: Given concerning trends in antibiotic resistance, phages have been increasingly explored as promising antimicrobial agents. However, a major problem with phage therapy is the overly high specificity of phages for their hosts, which is currently addressed by a personalized approach involving screening a bank of wild-type phages against each clinical isolate. To shorten this process, we propose that a focused library of synthetic phages could be rapidly selected for a member binding to a given clinical isolate. We created libraries of recombinant M13 phages expressing receptor-binding proteins based on the collective metagenome of inovirus phages, a diverse group whose members appear to infect nearly all bacterial phyla. Using two rounds of a pull-down selection, phage variants were identifi

SUBMITTER: Yang Y 

PROVIDER: S-EPMC12477597 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature