SIRT1 Expression in Human Gastrointestinal Tumors and Its Clinical Significance.

ABSTRACT:

Objective

This study aimed to investigate the expression levels of SIRT1 protein in gastric (GC), colon (CC), and rectal cancer (RC) tissues and patient plasma, analyze its correlation with clinicopathological features and prognosis, and preliminarily explore its relationship with the tumor immune microenvironment.

Methods

Plasma samples were collected from 198 gastrointestinal cancer patients (66 each of GC, CC, and RC) and 66 healthy volunteers. Additionally, cancerous and adjacent normal tissues were obtained from 45 of these patients (15 pairs for each cancer type). SIRT1 concentration in plasma was detected using Enzyme-Linked Immunosorbent Assay (ELISA). SIRT1 protein expression in tissues was examined using Immunohistochemistry (IHC) and Western Blotting. Bioinformati

SUBMITTER: Liu W 

PROVIDER: S-EPMC12477621 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature