Engineered dermal tissue constructs using mesenchymal stromal cells and TGF-β3-loaded electrospun dressings for stimulated wound healing process.

ABSTRACT: Transforming growth factor-beta 3 (TGF-β3) has been shown to promote wound healing by regulating key cellular processes. However, its clinical application is limited by the need for repeated dosing and by its labile nature, as TGF-β3 is sensitive to physiological fluctuations in temperature and pH, which can compromise its stability and efficacy. In this study, we developed a novel scaffold composed of poly(ε-caprolactone) and type I collagen as a matrix to immobilize calcium alginate capsules loaded with TGF-β3, called PCAT. This system enables localized delivery of the factor to the lesion site while preserving its bioactivity, positioning PCAT as an effective growth factor-release platform. In vitro characterization using human Wharton's jelly mesenchymal stromal cells (hWJ-MSCs) cultur

SUBMITTER: Lizarazo-Fonseca L 

PROVIDER: S-EPMC12477933 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature