Characterization of bacterial intrinsic transcription terminators identified with TERMITe - a novel method for comprehensive analysis of Term-seq data.

ABSTRACT: In recent years, Term-seq became a standard experimental approach for high-throughput identification of 3' ends of bacterial transcripts. It was widely adopted to study transcription termination events and 3' maturation of bacterial RNAs. Despite widespread utilization, a universal bioinformatics toolkit for comprehensive analysis of Term-seq sequencing data is still lacking. Here, we describe TERMITe, a novel method for the identification of stable 3' RNA ends based on bacterial Term-seq data. TERMITe works with data obtained from both currently available Term-seq protocols and provides robust identification of the 3' RNA termini. Unique features of TERMITe include the calculation of the transcription termination efficiency using matched RNA-seq data and the comprehen

SUBMITTER: Kosinski JG 

PROVIDER: S-EPMC12478363 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature