Physical confinement and phagocytic uptake induce directional migration.

ABSTRACT: Physical confinement is not routinely considered as a factor that influences phagocytosis, which is typically assayed in unconfined settings in vitro. BV-2 microglia-like cells were used to interrogate the impact of confinement on IgG-mediated phagocytosis side by side with unconfined cells. Confinement acted as a potent phagocytic driver, greatly increasing the fraction of phagocytic cells in the population compared to the unconfined setting. Arp2/3 complex and myosin II contributed to this effect. Remarkably, confinement partially rescued phagocytic uptake upon myosin II disruption. In addition, cells under confinement were partially resistant to the actin-depolymerizing drug cytochalasin D. Unexpectedly, we observed that bead uptake stimulated persistent migration, a process we t

SUBMITTER: Paulson SG 

PROVIDER: S-EPMC12478418 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature