From vineyard to genome: optimized enrichment and sequencing of Flavescence doree phytoplasma from grapevine samples.

ABSTRACT: Phytoplasmas are non-culturable obligate intracellular bacteria that cause considerable economic losses in agriculture. Genome sequencing provides crucial insights into their biology and vector dependence. However, genome studies on phytoplasmas are often hampered by their low abundance in naturally infected plants. Propagation in test plants is usually necessary but time-consuming and resource-intensive, especially for quarantine phytoplasmas such as the phytoplasma causing Flavescence dorée (FD), a serious threat to European viticulture. To overcome these challenges, we aimed to develop a protocol for efficient enrichment of phytoplasma DNA directly from field-collected samples, enabling genome sequencing using both Illumina and Oxford Nanopore Technologies platforms. We evaluated six sa

SUBMITTER: Kogej Zwitter Z 

PROVIDER: S-EPMC12479172 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature