Membrane proteomics and transcriptomic profiling analysis of hepatic stellate cells co-incubated with <i>Schistosoma japonicum</i> eggs.

ABSTRACT: Schistosomiasis has been recognized as the second most prevalent parasitic disease worldwide, following malaria. Schistosome eggs can persist for extended periods within host hepatic tissues, leading to hepatic fibrosis primarily through the activation of hepatic stellate cells (HSCs). However, the mechanisms by which egg-secreted products modulate the activation of HSCs remain incompletely elucidated. In this study, purified Schistosoma japonicum (S. japonicum) eggs (Egg group) or corresponding unused medium (Control group) were placed in the upper chamber of a Transwell system, with HSCs cultured in the lower chamber. Following co-culture, HSCs surface proteins were eluted and subsequently analyzed by mass spectrometry. Protein identities were determined by matching spectra

SUBMITTER: Dong B 

PROVIDER: S-EPMC12479499 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature