Emergence of tigecycline-resistant <i>Raoultella ornithinolytica</i> with <i>tet</i>(X)-carrying plasmid from swine wastewater in China.

ABSTRACT:

Background

The increasing prevalence of antibiotic resistance genes (ARGs), such as the plasmid-mediated tigecycline-modifying enzyme tet(X), significantly hinders the treatment of infectious diseases in humans and animals. Livestock wastewater contributes to the transmission of these ARGs.

Methods

Between June 2023 and December 2024, 140 wastewater samples from 15 swine farms in Shandong, China, were screened for tet(X)-positive strains using PCR and 16S rRNA sequencing. Raoultella ornithinolytica SD8 was assessed for antimicrobial susceptibility, plasmid stability, conjugation, fitness cost, and pathogenicity in a BALB/c mouse model. Furthermore, this strain was subjected to whole-genome sequencing.

Results

tet(X4) was found to be located

SUBMITTER: Jia Q 

PROVIDER: S-EPMC12479518 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature